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目的:评价EB病毒潜伏膜蛋白1(LMP-1)和鼻咽癌上皮间质转化相关分子与颈淋巴结转移关系。方法:88例鼻咽癌活检标本依据病理检查或MRI检查分成病理转移组(18例)、MRI转移组(40例)和无转移组(30例)。免疫组织化学检测鼻咽癌组织中潜伏膜蛋白1(LMP-1)、STAT3、Twist、E-cadherin和Vimentin的表达情况。结果:88例鼻咽癌组织中,LMP-1阳性表达见于35例,阳性率为38.7%。病理转移组、MRI转移组和无转移组的LMP-1阳性率分别为38.9%(7/18)、47.5%(19/40)和30.0%(9/30),3组间差异无统计学意义。LMP-1表达分别与Twist和Vimentin表达呈正相关(r=0.276和0.282,均P<0.01),与STAT3和E-Cadherin表达无相关性。88例鼻咽癌组织中STAT3、Twist和Vimentin阳性率以及E-Cadherin异常表达率分别64.8%(57/88)、54.5%(48/88)、20.0%(22/88)和60.2%(53/88)。STAT3、Twist和Vimentin阳性率以及E-Cadherin异常表达率在病理转移组、MRI转移组和无转移组间差异均分别有统计学意义(均P<0.05)。STAT3表达分别与Twist和Vimentin表达均呈正相关性(r=0.712和0.316,均P<0.01)。结论:EMT在鼻咽癌转移过程中起着重要作用。LMP-1可能仅作为上游影响因素之一间接影响EMT的发生,但与颈部淋巴结转移可能无直接相关性。
OBJECTIVE: To evaluate the relationship between Epstein-Barr virus latent membrane protein 1 (LMP-1) and cervical epithelial-mesenchymal transition related molecules and cervical lymph node metastases. Methods: Eighty-eight nasopharyngeal carcinoma biopsy specimens were divided into pathologic group (18 cases), MRI metastatic group (40 cases) and non-metastasis group (30 cases) according to pathological examination or MRI examination. Immunohistochemistry was used to detect the expression of LMP-1, STAT3, Twist, E-cadherin and Vimentin in nasopharyngeal carcinoma. RESULTS: In 88 cases of nasopharyngeal carcinoma, the positive expression of LMP-1 was found in 35 cases, and the positive rate was 38.7%. The positive rates of LMP-1 were 38.9% (7/18), 47.5% (19/40), and 30.0% (9/30) in pathological metastasis group, MRI metastasis group, and non-metastasis group, respectively. There was no statistical difference between the three groups. significance. LMP-1 expression was positively correlated with Twist and Vimentin expression (r=0.276 and 0.282, both P<0.01), but not with STAT3 and E-Cadherin expression. The positive rate of STAT3, Twist and Vimentin and the abnormal expression rate of E-Cadherin in 88 cases of nasopharyngeal carcinoma were 64.8% (57/88), 54.5% (48/88), 20.0% (22/88) and 60.2% (53, respectively). /88). The positive rate of STAT3, Twist and Vimentin and the abnormal expression rate of E-Cadherin in the pathological metastasis group, MRI metastasis group and non-metastasis group were statistically significant (all P<0.05). The expression of STAT3 was positively correlated with the expression of Twist and Vimentin, respectively (r=0.712 and 0.316, both P<0.01). Conclusion: EMT plays an important role in the metastasis of nasopharyngeal carcinoma. LMP-1 may only affect the occurrence of EMT indirectly as one of the upstream factors, but may not be directly related to cervical lymph node metastasis.