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目的:预处理对木质纤维素降解的影响。方法:从土壤中分离筛选到高纤维素酶活的黏细菌菌株So ce sh1008。该菌具有CMC酶活(CMCase)及微晶纤维素酶活性。研究NaOH联合黏细菌降解盐蒿、稻草、棉花秸秆和甘蔗渣四种木质纤维素的情况。结果:碱(2%NaOH)-黏细菌处理的方法优于黏细菌-碱的方法,其中降解棉花秸秆降解效果最明显,以5.0g木质纤维素为原料,其最终干重损失达2.1g,溶液中总糖含量和还原糖含量均值分别为12.8 mg/mL和0.93 mg/mL。酵母菌发酵产乙醇的研究结果表明,最佳发酵时间为47h,碱-黏细菌甘蔗渣降解液发酵效果最好,乙醇产出达6.0%。结论:黏细菌联合2%NaOH能有效降解甘蔗渣,提高乙醇产量。
Objective: The effect of pretreatment on the degradation of lignocellulose. Methods: Highly cellulase-producing myxobacteria strain So ce sh1008 was isolated from soil. The bacteria have CMCase activity (CMCase) and microcrystalline cellulose activity. Study on the degradation of four kinds of lignocelluloses including Artemisia ordosica, straw, cotton stalks and bagasse by NaOH combined with viscous bacteria. Results: Alkaline (2% NaOH) - mycobacterial treatment was superior to mycobacteria - alkali. The degrading effect of cotton straw was the most obvious. The final dry weight loss was 2.1g with 5.0g lignocellulose as raw material, The average total sugar content and reducing sugar content of the solution were 12.8 mg / mL and 0.93 mg / mL, respectively. The results of yeast fermentation ethanol production showed that the best fermentation time was 47h, alkali-viscous bacteria bagasse degradation solution fermentation best, ethanol output reached 6.0%. Conclusion: Viscous bacteria combined with 2% NaOH can effectively degrade bagasse and increase ethanol production.