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本文用免疫细胞化学法(ICC)和放射免疫测定(RIA),对成年大白鼠小肠内血管活性肠肽(VIP)、P 物质(SP)、亮氨酸-脑啡肽(L-Enk)和生长抑素(SOM)进行定性、定位和定量的分析。ICC 结果显示4种肽在小肠分布于肠神经丛和肠道内分泌细胞中。VIP、SP 和 L-Enk 免疫反应性神经元胞体位于肠神经节内,未见 SOM 能神经元。VIP 和 SP 能神经纤维分布于小肠各层。L-Enk 能纤维主要分布在肌间神经丛和环肌层。SOM 免疫阳性纤维主要见于肌间神经丛。除VIP 外,其余3种肽均见于相应的内分泌细胞,后者位于肠绒毛上皮和肠腺上皮细胞之间。4种肽的 RIA(ng/g)分别为:VIP 150.8;SP 60.7;L-Enk 14.1;SOM 4.2。其含量与 ICC阳性反应结构的分布密度相吻合,以 VIP 最高,SP、L-Enk 次之,SOM 仅少量存在于小肠。
In this study, immunocytochemistry (ICC) and radioimmunoassay (RIA) were used to detect the intestinal vasoactive intestinal peptide (VIP), substance P (SP), leucine-enkephalin Somatostatin (SOM) qualitative, positional and quantitative analysis. The results of ICC showed that four kinds of peptides are distributed in the small intestine in the enteric plexus and intestinal endocrine cells. VIP, SP, and L-Enk immunoreactive neuronal soma were located in the enteric ganglia and no SOM neurons were found. VIP and SP nerve fibers distributed in the small intestine layers. L-Enk fibers mainly in the myenteric plexus and the ring muscle. SOM-positive fibers are found mainly in the myenteric plexus. In addition to the VIP, the remaining three peptides are found in the corresponding endocrine cells, the latter between the intestinal villi epithelial cells and glandular epithelial cells. The RIA (ng / g) for the four peptides were: VIP 150.8; SP 60.7; L-Enk 14.1; SOM 4.2. The content was consistent with the distribution density of ICC positive reaction structure, which was highest in VIP, followed by SP and L-Enk, while SOM only existed in small intestine.