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待检样品中蛋白质的非特异性吸附是传感器检测临床血清亟待解决的问题。该文提出了一种在传感器表面制备抗蛋白质非特异性吸附涂层的方法,以二乙烯基砜(DVS)作为偶联剂,将天然氨基酸分子键合在经3-氨丙基三乙氧基硅烷(APTES)修饰的传感器表面,制备出一类新型抗垢涂层。通过蛋白吸附实验对多种氨基酸进行了筛选,并结合接触角的变化对键合条件进行了优化,并采用X射线光电子能谱(X-ray photoelectron spectroscopy,XPS)表征了制备的涂层。结果表明,使用赖氨酸(50 mmol/L)在p H9.5的条件下制备的抗垢涂层具有最优的抗蛋白非特异性吸附能力。该文采用该方法在生物膜干涉传感器(Biolayer Interferometry,BLI)表面制备了抗垢涂层,并以森林脑炎为例,对森林脑炎患者血清进行检测,得到测量结果与临床ELISA检测结果相吻合,表明该文所建立的方法可以应用于血清样品的快速定量检测。该文构建的基于天然氨基酸的抗垢涂层,制备简单,成本低廉,而且容易实现抗垢与特异性临床诊断的双功能,具有临床应用潜力。
Non-specific adsorption of protein in the sample to be tested is a problem to be solved by the sensor in detecting clinical serum. In this paper, a method of preparing anti-protein non-specific adsorption coating on the surface of sensor is proposed. Divinyl sulfone (DVS) is used as coupling agent to bind natural amino acid molecules to 3- Silane (APTES) modified sensor surface to prepare a new type of anti-scale coating. A variety of amino acids were screened by protein adsorption experiments, and the bonding conditions were optimized with the change of contact angle. The prepared coatings were characterized by X-ray photoelectron spectroscopy (XPS). The results show that the anti-scale coating prepared with lysine (50 mmol / L) at p H9.5 has the best nonspecific anti-protein adsorption capacity. In this paper, this method interferometric sensor (Biolayer Interferometry, BLI) anti-fouling surface coating prepared in the biofilm, and an example forest encephalitis, tick-borne encephalitis for serum detection, measurement results with the clinical results with ELISA test Coincide, indicating that the method established in this paper can be applied to the rapid quantitative detection of serum samples. The anti-fouling coating based on natural amino acids constructed in this paper has the advantages of simple preparation, low cost and easy bi-function of anti-fouling and specific clinical diagnosis.