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以非洲紫罗兰叶片和叶柄为外植体,以MS为基本培养基,比较了外植体类型、接种方式和植物生长调节剂配比对不定芽发生的影响,并初步探讨了试管内开花所需环境条件。结果表明:非洲紫罗兰叶片为较好的外植体类型,可以直接诱导产生不定芽,培养基配方为MS+6-BA0.5 mg/L+NAA0.1mg/L时不定芽诱导率较高;且接种方式对不定芽的诱导有重要影响,以叶片背面向下较好;活性炭有利于试管苗的生长;非洲紫罗兰试管内开花的适宜环境条件是光照时间14h/d,光照强度4 000lx,白天温度28℃,夜间温度20℃。
Taking the leaves and petiole of African violets as explants and MS as the basic medium, the effects of explant types, inoculation methods and plant growth regulators on the adventitious buds were compared, and the requirements for flowering in vitro Environmental conditions. The results showed that the leaves of P. violaceum were the best explant types, which could induce adventitious buds directly. The induction rate of adventitious buds was higher when the medium recipe was MS + 6-BA0.5 mg / L + NAA0.1 mg / L; And the inoculation mode had an important influence on the induction of adventitious buds, with the back of the leaves descending better; the activated carbon was conducive to the growth of test-tube seedlings; the suitable environmental conditions for the flowering of Viola yunnanensis were illumination time 14h / d, light intensity 4000 Ix, Temperature 28 ℃, night temperature 20 ℃.