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乳大鼠心肌剪成１ｍｍ３大小块，用１％胰蛋白酶消化制成每升５×１０８细胞悬液，２４孔板每孔接种１ｍＬ，以含小牛血清１６．６％的Ｅａｇｌｅ培养基（Ｅａｇｌｅ′ｓｍｅｄｉｕｍ）培养液培养７２ｈ后７１孔细胞分为正常培养液培养组及含普罗帕酮０．３１３，０．６２...
The heart of the rat was cut into 1mm3 size pieces, digested with 1% trypsin to make a suspension of 5 × 108 cells per liter. In a 24-well plate, 1mL was inoculated into each well and cultured in Eagle’s medium containing 16.6% fetal bovine serum ’Smedium) culture medium 72h after 72-hole cells were divided into normal culture medium and containing propafenone 0.313, 0.62 ...