论文部分内容阅读
目的对2014—2015年淮安市艾滋病病毒(HIV)抗体筛查阳性标本的确证结果进行分析,为艾滋病防治工作提供依据。方法对2014—2015年淮安市353例HIV抗体筛查阳性标本采用蛋白免疫印迹试验(WB)进行确证。结果 353例筛查阳性样本经WB确证,阳性257例,占72.80%;阴性62例,占17.56%;不确定34例,占9.63%。不同机构筛查阳性样本的确证阳性率差异具有统计学意义(χ~2=140.479,P=0.000),其中采供血机构的确证阳性率最低,为11.54%。在确证阳性的标本中,出现7条以上带型的225例,占87.55%;gp160和gp120条带出现率最高为100.00%,其次是p24,出现率为98.44%。不确定的样本中,p24出现单一条带最多,占44.12%;22例成功随访,其中有7例HIV抗体转阳。结论通过WB确证实验可以排除筛查实验中的假阳性,对于不确定的样本一定要加强随访。
Objective To analyze the confirmatory results of positive HIV antibody screening samples from 2014 to 2015 in Huai’an City and provide the basis for AIDS prevention and control. Methods 353 cases of HIV antibody screening positive samples from 2014 to 2015 in Huaian city were confirmed by Western blotting (WB). Results A total of 353 positive samples were screened by WB, of which 257 were positive, accounting for 72.80%; 62 were negative, accounting for 17.56%; 34 were uncertain, accounting for 9.63%. The positive rate of positive screening by different institutions was statistically significant (χ ~ 2 = 140.479, P = 0.000). The positive rate of confirmed blood sampling was 11.54%. Among the confirmed positive samples, there were 225 cases with more than 7 bands, accounting for 87.55%. The highest incidence of gp160 and gp120 bands was 100.00%, followed by p24, with an incidence rate of 98.44%. In the indeterminate sample, p24 showed the most single band, accounting for 44.12%. Twenty-two patients were followed up successfully, of which 7 were HIV positive. Conclusion The false positives in screening experiments can be excluded by WB confirmatory experiments, and the follow-up must be strengthened for uncertain samples.