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目的 为了探讨建立一种简便而可靠的恙虫病立克次体增殖方法， 以得到较大量的恙虫病立克次体。方法 采用了以 Ｖｅｒｏ － Ｅ６ 细胞增殖恙虫病立克次， 并且对 Ｖｅｒｏ － Ｅ６ 感染细胞的培养条件进行了改进。结果 成功地使恙虫病立克次体在 Ｖｅｒｏ － Ｅ６ 细胞中得到大量增殖。结论 改进后的 Ｖｅｒｏ － Ｅ６ 细胞培养法可以获得大量的恙虫病立克次体， 该方法既经济又适用。
Objective To explore a simple and reliable method for Rickettsia tsutsugamushi proliferation, in order to get a larger amount of Rickettsia tsutsugamushi. Methods Rickettsia tsutsugamushi was amplified with Vero - E6 cells and the culture conditions of Vero - E6 infected cells were improved. As a result, Rickettsia tsutsugamushi was successfully proliferated in Vero - E6 cells. Conclusion The improved Vero - E6 cell culture method can obtain a large number of Rickettsia tsutsugamushi, this method is both economical and applicable.