常用的分离单核细胞的方法,是依据其粘附功能,或依据其密度特性。利用密度的有淘析器法。利用粘附功能的有未处理的塑料法、细胞微渗出物或胎牛血清(FBS)包被的烧瓶法等。但由于设备贵、费时多、细胞活性差、产量低及血小板污染等问题,使制备大量人新鲜外周血单核细胞的方法受到了限制。根据单核细胞可通过纤维结合素(FN)受体,依赖于镁的机理,结合于经 Commonly used methods for isolation of monocytes are based on their adhesion function or on the basis of their density characteristics. Use of the density of the elutriation method. Using the adhesion function of untreated plastic method, cell micro-exudate or fetal bovine serum (FBS) coated flask method. However, due to equipment expensive, time-consuming, poor cell activity, low yields and platelet contamination and other issues, so that a large number of people preparing fresh peripheral blood mononuclear cells is limited. According to monocytes through the fibronectin (FN) receptor, dependent on the mechanism of magnesium, binding to the