论文部分内容阅读
为开发碧桃转录组微卫星信息,利用454高通量测序技术,对其花瓣转录组序列进行SSR位点发掘,结果发现含SSR的序列4 705条,共得到5 668个SSR,平均每3.49 kb出现1个SSR。微卫星序列主要以三碱基重复为主,约占总数的42.66%。笔者共发现516种碱基重复基元,所占比例最高的为(AG/CT)n(18.34%),其次是(AAG/CTT)n(12.42%)。微卫星多为重复长度小于20 bp的短序列,长度大于20 bp的微卫星仅占总数的12.13%。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关(P<0.05),相关系数为-0.246。
In order to develop the microsatellite information of Tachyosanthus transcriptome, 4555 high-throughput sequencing techniques were used to explore the SSR loci of its petal transcriptome sequence. It was found that there were 4 705 SSR-containing sequences with 5 668 SSRs averaged every 3.49 kb 1 SSR appears. Microsatellite sequences mainly in three base repeat, accounting for 42.66% of the total. A total of 516 base repeat motifs were found, with the highest proportion being (AG / CT) n (18.34%), followed by (AAG / CTT) n (12.42%). Most microsatellites are short sequences with repetitive length of less than 20 bp, and microsatellites with length greater than 20 bp account for only 12.13% of the total. The study also found a significant negative correlation (P <0.05) between the frequency and length of the petals of the blue peach petals, with a correlation coefficient of -0.246.