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目的:本研究旨在观察丹酚酸B对体外培养新生大鼠海马神经元的影响,为丹酚酸B对中枢神经系统的作用提供实验依据。方法:体外培养新生大鼠海马神经元,神经元微管相关蛋白-2(MAP-2)免疫荧光染色对培养细胞进行鉴定,实验分为对照组和给药组(终浓度分别为5 mg/L和10 mg/L),荧光显微镜下观察细胞形态,MTT比色法检测丹酚酸B对海马神经细胞的活性,细胞核染色(DAPI)观察丹酚酸B对海马神经细胞增殖的影响。结果:免疫荧光染色显示细胞呈MAP-2阳性,丹酚酸B作用7 d后,能明显促进海马神经细胞的存活与增殖,且10 mg/L的丹酚酸B作用更明显。结论:丹酚酸B能明显促进海马神经细胞的存活与增殖,提示其具有改善脑功能的重要作用。
OBJECTIVE: To investigate the effect of salvianolic acid B on cultured hippocampal neurons of neonatal rats in vitro, and to provide experimental evidence for the effect of salvianolic acid B on the central nervous system. Methods: Cultured neonatal rat hippocampal neurons were cultured in vitro, and neuronal microtubule-associated protein-2 (MAP-2) immunofluorescence staining was used to identify cultured cells. The experiment was divided into control group and treatment group (final concentrations were 5 mg / L and 10 mg / L). The cell morphology was observed under a fluorescence microscope. The activity of salvianolic acid B in hippocampal neurons was detected by MTT colorimetric assay. The effect of salvianolic acid B on the proliferation of hippocampal neurons was observed by nuclear staining (DAPI). Results: Immunofluorescence staining showed that MAP-2 cells were positive. After treated with salvianolic acid B for 7 days, the survival and proliferation of hippocampal neurons were significantly enhanced. The effect of salvianolic acid B at 10 mg / L was more obvious. Conclusion: Salvianolic acid B can significantly promote the survival and proliferation of hippocampal neurons, suggesting that it has an important role in improving brain function.