本文报道用反相高效液相色谱法测定薄盖灵芝发酵菌丝体中主要核苷及其碱基的含量。菌丝体用50%乙醇超声提取,脱脂后通过十八烷基键合相柱,用磷酸盐缓冲液(pH 6.98)—四氢呋喃(100:1)为流动相,对甲基苯磺酸为内标,峰面积内标法定量。方法灵敏、快速、重现性好,与薄层紫外法比较结果一致。 This paper reports the determination of main nucleosides and their bases in the mycelia of Ganoderma lucidum by RP-HPLC. The mycelia were extracted with ultrasonic 50% ethanol, deblistered and passed through an octadecyl bonding phase column. The mobile phase was phosphate buffer (pH 6.98)-tetrahydrofuran (100:1), and the p-toluenesulfonic acid was used as the mobile phase. Standard, peak area internal standard method quantification. The method is sensitive, rapid, and reproducible, and is consistent with the thin layer UV method.