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背景:利用D-半乳糖中毒法建立动物衰老模型已经多年,并在抗衰老药物的筛选和研究开发方面发挥了重要作用,但其衰老机制仍然不甚清楚。国内学者倾向于自由基衰老学说,但也缺乏相应的数据支持。目的:D-半乳糖皮下注射致Wistar大鼠亚急性衰老模型衰老的变化。设计:随机对照的实验研究。地点、材料和干预:本实验在北京解放军空军总医院动物实验中心完成。20只雄性大鼠随机分为两组,每组10只。对照组皮下注射生理盐水,10mL/kg,1次/d;衰老模型组皮下注射D-半乳糖48mg/kg,1次/d。各组大鼠均连续给药42d,采血后分别测定各项衰老指标。主要观察指标:衰老对Wistar大鼠体质量、胸腺、脾脏系数、血象指标、血清超氧化物歧化酶(superoxidedismutase,SOD)活性、过氧化脂质(lipidperoxidation,LPO)活性、血糖、胆固醇、三酰甘油浓度的影响。结果:与对照组比较,衰老模型组体质量增加比例降低,胸腺明显萎缩,脏器系数减少(t=3.5,P<0.01),血清中SOD活性明显下降(t=3.06,P<0.01)。其他指标无明显变化。结论:D-半乳糖诱发的致大鼠亚急性衰老是探讨自由基衰老学说有用的实验动物造模技术。
Abstract BACKGROUND: The establishment of an animal aging model using D-galactose poisoning has been around for many years and has played an important role in the screening and research and development of anti-aging drugs. However, the mechanism of aging remains unclear. Domestic scholars tend to radical aging theory, but also lack the appropriate data support. OBJECTIVE: To observe the senescence of sub-acute aging model induced by D-galactose subcutaneous injection in Wistar rats. Design: Randomized controlled experimental study. Location, Materials and Interventions: This experiment was completed at the Animal Experimental Center of PLA General Hospital of PLA. Twenty male rats were randomly divided into two groups of 10 rats. The control group was subcutaneously injected with normal saline (10 mL / kg, once a day). The model group was injected subcutaneously with D-galactose 48 mg / kg once a day. The rats in each group were administrated continuously for 42 days, after which the indexes of aging were determined respectively. MAIN OUTCOME MEASURES: The effects of aging on body weight, thymus, spleen coefficient, blood index, serum superoxide dismutase (SOD) activity, lipid peroxide (LPO) activity, blood glucose, cholesterol, Effect of glycerol concentration. Results: Compared with the control group, the body weight of the aging model decreased, the thymus constricted, the organ coefficient decreased (t = 3.5, P <0.01) and the serum SOD activity decreased significantly (t = 3.06, P <0.01). Other indicators no significant change. Conclusion: D-galactose-induced subacute aging in rats is a useful experimental animal modeling technique for exploring the theory of free radical aging.