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[目的]分析杭州市2009年1月至2011年1月新甲型H1N1流感病毒血凝素(HA)的基因序列,探讨该病毒的遗传变异和分子特性。[方法]应用RT-PCR的方法从临床标本中扩增新甲型H1N1流感病毒HA基因片段并测序。利用DNAMAN和Sequencher4.7软件对HA基因序列进行分析,比较HA基因氨基酸亲缘系数并绘制进化树。[结果]成功地对15例新甲型H1N1病毒的HA基因进行扩增并测序,2009年1月至2011年1月新甲型H1N1流感病毒的HA基因的氨基酸序列与参考株具有高度的同源性,其亲缘系数为98.9%~100%,且HA基因氨基酸序列发生了一定的变异。[结论]2009年至2011年新甲型H1N1流感病毒的基因组HA序列与我国和其他国家的新甲型H1N1流感病毒具有高度的同源性,但HA基因的氨基酸序列发生了变异,可能与患者的病情有关,因此需密切关注新甲型H1N1流感病毒的流行情况。
[Objective] To analyze the gene sequence of hemagglutinin (HA) of the new influenza A (H1N1) virus in Hangzhou from January 2009 to January 2011 and to investigate the genetic variation and molecular characteristics of the virus. [Method] The HA gene fragment of influenza A (H1N1) virus was amplified from clinical samples by RT-PCR and sequenced. The HA gene sequences were analyzed by using DNAMAN and Sequencher4.7 software to compare the amino acid phylogenetic relationships of HA genes and draw the phylogenetic tree. [Results] The HA gene of 15 cases of new type A H1N1 virus were successfully amplified and sequenced. The amino acid sequence of the HA gene of the new influenza A (H1N1) virus from January 2009 to January 2011 was highly homologous to the reference strain The kinship coefficient was between 98.9% and 100%, and the amino acid sequence of HA gene had some variation. [Conclusion] The genomic HA sequence of the influenza A (H1N1) virus from 2009 to 2011 is highly homologous to the new influenza A (H1N1) virus in China and other countries. However, the amino acid sequence of the HA gene is mutated and may be related to the patients Of the disease, and therefore need to pay close attention to the epidemic of the new influenza A (H1N1) virus.