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采用日本血吸虫重组BCG-Sj26GST疫苗免疫BALB/c小鼠。免疫8周时,断头取血,取脾脏,取腹腔巨噬细胞。以淋巴细胞刺激指数(SI)反映细胞增殖能力,以NO释放量检测巨噬细胞吞噬活性,以ELISA试剂盒检测血清及脾淋巴细胞培养上清的白细胞介素2(IL-2)和干扰素-γ(IFN-γ)的含量。结果表明,rBCG-Sj26GST疫苗以 10~6CFU剂量经皮下免疫小鼠后,小鼠脾淋巴细胞增殖能力明显高于对照组、载体组和BCG组(均为P<0.05);小鼠腹腔巨噬细胞NO的含量明显高于对照组和载体组(均为P<0.01);小鼠血清IL-2的含量明显高于对照组(P<0.01)和载体组(P<0.05);而小鼠脾淋巴细胞培养上清IL-2的含量分别高于对照组44%、载体组48%和BCB组42%;小鼠血清IFN-γ的含量分别高于对照组20%、载体组19%和BCG组13%,均有升高趋势、结果提示,rBCG-Sj26GST疫苗能明显增强小鼠的免疫应答反应,其抗感染作用一方面与BCG本身的佐剂特性有关,另一方面与机体产生抗Sj26-GST特异性抗体有关。
BALB / c mice were immunized with recombinant Schistosoma japonicum BCG-Sj26GST vaccine. 8 weeks after immunization, decapitated blood, take the spleen, take peritoneal macrophages. Lymphocyte stimulation index (SI) was used to reflect the proliferation of macrophages. The phagocytic activity of macrophages was detected by NO release. The levels of interleukin 2 (IL-2) in serum and splenic lymphocyte culture supernatants and interferon -γ (IFN-γ) content. The results showed that the spleen lymphocyte proliferation ability of rBCG-Sj26GST vaccine was significantly higher than that of control group, vehicle group and BCG group (all P <0.05) after subcutaneous immunization with rBCG-Sj26GST vaccine at the dose of 10 ~ 6CFU. The content of NO in macrophages was significantly higher than that in control group and vehicle group (all P <0.01). The level of serum IL-2 in macrophages was significantly higher than that in control group and vehicle group (P < 0.05). The contents of IL-2 in the supernatant of splenic lymphocytes of mice were 44% higher than those in the control group, 48% in the carrier group and 42% in the BCB group, respectively. The levels of serum IFN-γ in the mice were higher than those in the control group The results showed that the rBCG-Sj26GST vaccine significantly enhanced the immune response in mice, and its anti-infective effect was related to the adjuvanticity of BCG itself , On the other hand with the body to produce anti-Sj26-GST specific antibodies.