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1986年,我们选取开花10天左右的菊花变异株,在无菌条件下,切取带有未萌动侧芽的茎段,接种在含2毫克/毫升6-BA+0.2毫克/毫升NAA+1毫克/毫升GA_3的MS培养基上(图1),每天光照12小时,光强度1500勒克斯,温度25—28℃,经5—7天出现黄绿色愈伤组织,待一个培养周期后,将愈伤组织切成小块转入增殖培养基培养,再转入含10毫克/毫升GA_3+3毫克/毫升6-BA的MS培养基,30—40天后分化出苗。苗高2—3厘米时,转入含0.3毫克/毫升NAA的MS培养基上诱导生根(图2)。3月下旬移入阳畦,5月下旬栽入大田,精
In 1986, we selected the chrysanthemum mutant which had been blossomed for about 10 days. Under the aseptic condition, the stems with unprotected lateral buds were excised and inoculated into the medium containing 2 mg / ml 6-BA + 0.2 mg / ml NAA + 1 mg / Ml GA_3 MS medium (Figure 1), light 12 hours a day, 1500 lux light intensity, temperature 25-28 ℃, 5-7 days appear yellow-green callus, to be a culture cycle, the callus Cut into small pieces and transferred to proliferation medium, and then transferred to MS medium containing 10 mg / ml GA_3 + 3 mg / ml 6-BA. 2-3 cm in height, transferred to MS medium containing 0.3 mg / ml NAA to induce rooting (Figure 2). In late March moved to the sunburns, late May planted fields, fine