论文部分内容阅读
采用荧光光谱分析法,研究了饮用水消毒副产物三氯甲烷、三溴甲烷与人血丙种球蛋白之间的相互作用.结果表明,三氯甲烷对人血球蛋白产生动态荧光猝灭,而三溴甲烷对人血球蛋白的内源荧光有静态猝灭作用.Fe3+、Al3+和聚丙烯酰胺的加入也使人血丙种球蛋白的荧光强度降低,结合的强弱依次为:聚丙烯酰胺>Fe3+>Al3+.在二元体系的基础上,进一步研究了Fe3+、Al3+和聚丙烯酰胺对CHBr3-人血丙种球蛋白结合作用的影响.结果表明,Fe3+、Al3+的存在使CHBr3-人血丙种球蛋白的结合常数呈现降低的趋势,聚丙烯酰胺对其基本无影响.
Fluorescence spectroscopy was used to study the interaction between trichloromethane and tribromomethane, a byproduct of drinking water disinfection, and human gamma globulin.The results showed that trichloromethane produced dynamic fluorescence quenching of human hemoglobin, Methyl bromide has a static quenching effect on the endogenous fluorescence of human hemoglobin, and the addition of Fe3 +, Al3 + and polyacrylamide also decreases the fluorescence intensity of human gamma-globulin, the order of binding is: polyacrylamide> Fe3 + Al3 + .Based on the binary system, the effect of Fe3 +, Al3 + and polyacrylamide on the binding of CHBr3-human gamma globulin was further studied.The results showed that the presence of Fe3 + and Al3 + The binding constant showed a decreasing tendency, polyacrylamide had no effect on it.