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背景:MBL基因据认为在人类抗结核(TB)感染天然免疫应答中发挥作用。目的:探讨MBL基因多态性与中国汉族人群结核感染关联的可能性。设计:共有152名男性肺结核病人和293名健康男性对照纳入本研究。联合应用引物序列特异性PCR(PCR-SSP)和序列特异性寡核苷酸探针杂交(PCR-SSOP)方法对MBL基因六个单核苷酸多态性(SNPs)位点(A/B,A/C,A/O,H/L,Y/X和P/Q)进行基因型或单倍体型在病例组和对照组的分布进行比较,并进行非条件logistic回归分析。结果:当单独考虑时,5个位点的基因型和单倍体型与发病均无显著相关。然而,当重新分组后,和YA组相比,编码低水平MBL的XB单倍体组在病例组中有很高的出现频率(OR=1.57,95%CI:1.02-2.41, P<0.05)结论:虽然低水平XB单倍型组在中国汉族人群肺结核感染中为一较弱的危险因素,但MBL多态性和肺结核之间的关联没有观察到令人信服的证据。
Background: The MBL gene is thought to play a role in the innate immune response to human anti-TB (TB) infection. Objective: To explore the possibility of MBL gene polymorphism associated with tuberculosis infection in Chinese Han population. Design: A total of 152 male pulmonary tuberculosis patients and 293 healthy male controls were included in the study. The combination of primer pair specific PCR (PCR-SSP) and sequence specific oligonucleotide probe hybridization (PCR-SSOP) was used to detect the polymorphisms of six single nucleotide polymorphisms (SNPs) , A / C, A / O, H / L, Y / X and P / Q) were compared between the case group and the control group, and non-conditional logistic regression analysis was performed. RESULTS: Genotypes and haplotypes at 5 sites were not significantly associated with disease when considered individually. However, when reprogrammed, the haplotype XB haplotypes encoding low levels of MBL had a high frequency of occurrence in the case group (OR = 1.57, 95% CI: 1.02-2.41, P <0.05) as compared to the YA group Conclusions: Although low-level XB haplotypes are a weaker risk factor for tuberculosis in Chinese Han populations, no convincing evidence is observed for the association between MBL polymorphisms and tuberculosis.