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采用β-环糊精作为手性选择剂,使用微乳电动毛细管色谱方法在12min内成功进行氰戊菊酯对映异构体的手性拆分.对拆分的电泳条件,如缓冲溶液的组成、浓度、pH、微乳的组成及浓度、环糊精浓度等进行了讨论及优化.结果表明,在分离电压为20 kV,选用57 cm(有效长度50 cm)×75μm内径的石英毛细管,β-环糊精的浓度为30 mmol/L,缓冲溶液pH=6.5(5 mmol/L磷酸二氢钠+10 mmol/L硼砂溶液),以40 mmol/L SDS+ 30 mmol/L正庚烷+300 mmol/L正丁醇为微乳,在25℃分离,可使氰戊菊酸的4个对映体基线分离.
Chiral resolution of fenvalerate enantiomers was successfully performed within 12 min using β-cyclodextrin as a chiral selector, and electrophoretic conditions for resolution such as buffer solution Composition and concentration, pH, the composition and concentration of microemulsion and the concentration of cyclodextrin were discussed and optimized.The results showed that when the separation voltage was 20 kV, a 57 cm (effective length 50 cm) × 75 μm ID quartz capillary tube was used, The concentration of β-cyclodextrin was 30 mmol / L, the buffer solution was pH 6.5 (5 mmol / L sodium dihydrogen phosphate + 10 mmol / L borax solution), 40 mmol / L SDS + 30 mmol / 300 mmol / L n-butanol is a microemulsion, separated at 25 ° C, allowing the enantiomeric separation of the four enantiomers of fenvalerate.