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目的 :探讨慢性低O2 高CO2 条件下脑烯醇化酶 (NSE)、蛋白S10 0、超微结构等改变及川芎嗪对其影响。方法 :将SD大鼠分为正常对照组 (A组 ) ,低O2 高CO2 组 (B组 ) ,低O2 高CO2 +川芎嗪组 (C组 ) ,采用免疫组织化学、透射电镜方法 ,观察平均肺动脉压 (mPAP)、颈动脉压 (mCAP)、血清NO浓度、脑NSE、S10 0变化及脑超微结构等改变。结果 :①B组mPAP明显高于A组 ,C组低于B组 ,三组间mCAP无明显差异。②B组血清NO浓度低于A组 (P <0 .0 1) ,C组血清NO浓度高于B组 (P <0 .0 1)。③B组比A组NSE、S10 0吸光度值降低 ,C组比B组NSE、S10 0吸光度值增高。④B组神经元、神经胶质细胞水肿 ,C组神经细胞损害明显减轻。结论 :慢性低O2 高CO2 脑NSE、S10 0降低 ,川芎嗪对脑损害有保护作用。
Objective: To investigate the effects of ligustrazine on the changes of brain enolase (NSE), protein S10 0, ultrastructure in chronic hypoxia and hypercapnia. Methods: SD rats were divided into normal control group (group A), low O2 high CO2 group (group B), low O2 high CO2 + ligustrazine group (group C). Immunohistochemistry and transmission electron microscopy were used to observe the average Pulmonary artery pressure (mPAP), carotid artery pressure (mCAP), serum NO concentration, brain NSE, S10 0 changes and brain ultrastructure. Results: ① The mPAP in group B was significantly higher than that in group A, while that in group C was lower than that in group B. There was no significant difference in mCAP between the three groups. ② The NO concentration in serum of group B was lower than that of group A (P <0.01). The concentration of NO in group C was higher than that of group B (P <0.01). ③ In group B, NSE and S10 0 absorbance decreased compared with group A, and NSE and S10 0 absorbance increased in group C compared with group B. ④B group neurons, glial cell edema, C group of nerve cell damage was significantly reduced. Conclusion: Chronic hypoxia and hypercapnia brain NSE, S10 0 decreased, tetramethylpyrazine has a protective effect on brain damage.