教学实验中,常使用大量培养基。而制造培养基的牛肉膏及蛋白胨等价格昂贵,且都是一次性使用,消耗很大。为此,摸索培养基回收重复使用问题,具有一定的意义。经实验证明,普通肉汤及普通琼脂均可多次重复使用。现将方法介绍如下: 方法: (一)普通肉汤的回收应用:(以大肠杆菌为例)。 1.将大肠埃希氏菌(卫生部生物制品检定所44348接种于普通琼脂平板上, Teaching experiment, often use a large number of medium. The manufacturing of beef extract and peptone and other expensive, and are one-time use, consume a lot. For this reason, to explore the recycling of medium recycling problems, has a certain significance. The experiment proved that ordinary broth and ordinary agar can be repeatedly used. The method is introduced as follows: Method: (a) ordinary broth recovery application: (to E. coli, for example). 1. Escherichia coli (Ministry of Health 44348 biological samples were inoculated on an ordinary agar plate,