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目的建立同时测定百乐眠胶囊中2,3,5,4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷、远志口山酮Ⅲ、3,6二芥子酰基蔗糖的高效液相色谱法。方法采用Agilent Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5?m);流动相:甲醇-乙腈-0.05%磷酸溶液(32∶8∶60);流速:1.0 m L·min?1;检测波长:320 nm。结果 2,3,5,4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷、远志口山酮Ⅲ和3,6二芥子酰基蔗糖线性范围分别为0.020 6~0.309?g,0.041 6~0.624?g,0.022 4~0.336?g,线性关系良好(r依次为0.999 6,0.999 4,0.999 7),平均回收率分别为94.63%,99.63%,98.11%(n=9),RSD分别为2.1%,1.1%,1.0%。结论该方法准确可靠,重复性好,为百乐眠胶囊的质量控制提供了依据。
OBJECTIVE To establish a method for the simultaneous determination of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, polyglucoside Ⅲ, 3,6-diaminoacylsucrose in Bailemian capsule Chromatography. The method was performed on an Agilent Eclipse XDB-C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase was methanol-acetonitrile-0.05% phosphoric acid solution (32:8:60) Detection wavelength: 320 nm. Results The linear ranges of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, fargesicolone Ⅲ and 3,6-diaminoacyl sucrose were 0.020 6-0.309 μg, The average recoveries were 94.63%, 99.63% and 98.11% (n = 9), respectively. The average recoveries were 0.999 6, 0.999 4 and 0.9999, respectively. The average recoveries were 0.041 6-0.624 μg and 0.022 4-0.336 μg, RSD were 2.1%, 1.1%, 1.0%. Conclusion The method is accurate and reliable with good repeatability and provides the basis for the quality control of Bailemian capsule.