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Purpose: To study the property of LTP in layers Ⅱ-Ⅳof the rats visual cortex at different postnatal days induced by pairing low-frequency stimulation at layer Ⅵwith post synaptic depolarization in order to explore the synaptic and cellular mechanism of experience-dependent plasticity in the visual cortex. Methods: Postsynaptic currents (PSCs) of layers Ⅱ-Ⅳin visual cortex slices of Wistar rats aged P0-29 d were recorded by patch-clamp whole cell recording method. Long-term potentiation (LTP) was induced by low-frequency stimulation (LFS) at 1Hz for 60-90 s. Each pulse of the LFS paired with depolarization of post-synaptic neurons to -20 mV.100μM APV, a kind of competitive N-methyl-d-aspartate (NMDA) receptor antagonist, was both applied to some slices to test the property of LTP. Results:1. The LTP incidence was very low before P10d (5/34) , and increased rapidly to the top at P15-24 d (17/28), then decreased sharply to 1/5 at P25-29 d, coinciding well with the critical period of plasticity of rat
Purpose: To study the property of LTP in layers II-IV of the rats visual cortex at different postnatal days induced by pairing low-frequency stimulation at layer VI with post synaptic depolarization in order to explore the synaptic and cellular mechanism of experience-dependent plasticity in the visual cortex. Methods: Postsynaptic currents (PSCs) of layers Ⅱ-Ⅳin visual cortex slices of Wistar rats aged P0-29 d were recorded by patch-clamp whole cell recording method. Long-term potentiation (LTP) was induced by low-frequency stimulation (LFS) at 1 Hz for 60-90 s. Each pulse of the LFS paired with depolarization of post-synaptic neurons to -20 mV. 100 μM APV, a kind of competitive N-methyl-d-aspartate (NMDA) receptor antagonist, was both applied to some slices to test the property of LTP. Results: 1. The LTP incidence was very low before P10d (5/34), and increased rapidly to the top at P15-24 d (17/28), then decreased sharply to 1/5 at P25-29 d, coinciding well with the critical peri od of plasticity of rat