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目的 研究重组人酸性成纤维细胞生长因子 (rh-a FGF)对兔创伤的促愈合作用。方法 采用兔背部刀割伤模型 ,将 rh-a FGF溶液隔日一次滴注于创面 ,用创面照像、透明膜描记称量法记录伤后第 4、8、1 2、1 6天创面面积 ,用注水法测量伤腔容积 ,伤后第 8、1 6天取创面组织 ,观察创面的病理学变化 ,包括肉芽组织生长与再上皮化情况。结果 rh-a FGF可明显加速兔皮肤创伤的愈合 ,使创面面积明显缩小 (P<0 .0 5 ) ,使伤腔容积明显减少 (P<0 .0 5 )。组织学检查 :rh-a FGF组创面伤后 8天成纤维细胞生长活跃、数量多 ,其毛细血管胚芽与成纤维细胞数量显著多于对照组 ;伤后 1 6天 ,创面收缩与再上皮化明显 ,新生上皮向创面中心爬行较快。结论 rh-a FGF对兔背部刀伤创面有明显的促修复作用。
Objective To investigate the effect of recombinant human acidic fibroblast growth factor (rh-a FGF) on wound healing in rabbits. Methods Rabbit dorsal knife incision model was established. The rh-a FGF solution was instilled into the wounds every other day. The area of wounds on the 4th, 8th, the 12th and the 16th day after wounding were recorded by wound photography and transparent membrane tracing method. Wound volume was measured by water instillation, wound tissue was collected on the 8th and 6th day after wounding, pathological changes were observed, including granulation tissue growth and re-epithelialization. Results rh-a FGF could significantly accelerate the healing of rabbit skin wounds and significantly reduce the wound area (P <0.05), resulting in a significant reduction in the volume of wound cavity (P <0.05). Histological examination: fibroblasts in rh-a FGF group had more active and more number of fibroblasts 8 days after wounding, and the number of fibroblasts and fibroblasts in rh-a FGF group was significantly more than that of control group. On day 16 after wounding, wound shrinkage and reepithelization were obvious , Newborn epithelium to the center of the wound crawling faster. Conclusion rh-a FGF has a significant promoting effect on the back wound wound of rabbits.