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目的:研究含重复序列(GAA)n·(TTC)n的菌体在传代培养中氨苄青霉素对其遗传突变的影响。方法:采用分子克隆、PCR等分子生物学实验技术。结果:在100mLLB液体培养基中加入100mg/mL氨苄青霉素35μL对重复序列有明显删减。结论:治疗与重复序列相关的神经系统遗传疾病可以通过促进重复序列的有效删减,将重复序列数目控制在阈值之内来完成。
OBJECTIVE: To study the effect of ampicillin on genetic mutation in subculturing mycelia containing repetitive sequence (GAA) n · (TTC) n. Methods: Molecular cloning, PCR and other molecular biology experiments. Results: 35μL of 100mg / mL ampicillin was added to 100mLLB liquid medium, and the repeats were significantly deleted. CONCLUSIONS: The treatment of inherited diseases of the nervous system associated with repetitive sequences can be accomplished by facilitating the efficient deletion of repetitive sequences and controlling the number of repetitive sequences within the threshold.