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目的研究新合成化合物(顺)-3-(氯代亚甲基)-5-氟-硫色满-4-酮(CFTK)和(顺)-3-(氯代亚甲基)-5-甲基-硫色满-4-酮(CMTK)对人乳腺癌MCF-7细胞、人肝癌HepG-2细胞、人结肠癌LS174T细胞、人肺癌A549细胞、人黑色素瘤A375细胞、人肾癌786-O细胞、人慢性粒细胞白血病K562细胞、人系髓样白血病U937细胞、小鼠肉瘤S180细胞的抗肿瘤活性,为深入研究CFTK和CMTK抗肿瘤作用提供实验依据。方法以不同浓度的CFTK和CMTK作用于9种癌细胞,应用改良的MTT法检测CFTK和CMTK对9种癌细胞生长的抑制作用。结果通过检测以上细胞的增殖情况,得出CFTK对9种癌细胞的半数抑制浓度(IC50)在(6.32±1.52)μmol.L-1~(19.8±4.08)μmol.L-1之间,CMTK对9种癌细胞的IC50在(7.16±0.76)μmol.L-1~(18.2±5.28)μmol.L-1之间;与同浓度的顺铂(CDDP)相比,具有明显的抗肿瘤活性。结论 CFTK和CMTK能够明显抑制肿瘤细胞的生长,具有极高的体外抗肿瘤活性,为进一步研究开发提供依据。
OBJECTIVE To investigate the effect of novel cis-3-chloro-5-fluoro-thiochroman-4-one (CFTK) and (cis) -3- (chloromethylene) -5- Methyl-thiochroman-4-one (CMTK) on human breast cancer MCF-7 cells, human hepatocellular carcinoma HepG-2 cells, human colon cancer LS174T cells, human lung carcinoma A549 cells, human melanoma A375 cells, human renal carcinoma 786 O cells, human chronic myeloid leukemia K562 cells, human myeloid leukemia U937 cells, and mouse sarcoma S180 cells for providing experimental evidence for further study of antitumor effects of CFTK and CMTK. Methods Nine kinds of cancer cells were treated with different concentrations of CFTK and CMTK. The inhibitory effects of CFTK and CMTK on the growth of nine kinds of cancer cells were tested by MTT assay. Results The IC50 values of CFTK in nine kinds of cancer cells were (6.32 ± 1.52) μmol.L-1 ~ (19.8 ± 4.08) μmol.L-1, and CMTK The IC50 of the nine kinds of cancer cells was (7.16 ± 0.76) μmol.L-1 ~ (18.2 ± 5.28) μmol.L-1. Compared with the same concentration of cisplatin (CDDP), the IC50 had obvious anti-tumor activity . Conclusion CFTK and CMTK can significantly inhibit the growth of tumor cells, with high anti-tumor activity in vitro, to provide a basis for further research and development.