论文部分内容阅读
目的研究碘缺乏和甲状腺功能减退对大鼠仔鼠小脑cAMP反应元件结合蛋白(CREB)表达的影响。方法分别选用低碘饲料及丙基硫尿嘧啶(propylthiouracil,PTU)饮水诱导建立低碘及甲状腺功能减退Wistar大鼠动物模型,按体重随机分成对照组、甲状腺功能减退组(5mg/LPTU组、15mg/LPTU组)和碘缺乏组,出生后第14天(PN14)、PN21、PN28、PN42时,测定仔鼠小脑重量,PN7、PN14、PN21、PN28、PN42时取小脑组织进行免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)染色,用图像分析方法分析大鼠仔鼠小脑皮质CREB表达情况。结果PN14、PN21、PN28、PN42时,碘缺乏组小脑重量显著低于对照组和甲状腺功能减退组(P<0.05)。PN7时,各组之间小脑组织CREB平均积分光密度值差别不明显,而PN14,PN21,PN28和PN42时,5、15mg/LPTU组和碘缺乏组平均积分光密度值均低于对照组(P<0.05)。结论碘缺乏和甲状腺功能减退可降低大鼠仔鼠小脑组织CREB表达,影响神经系统的发育。
Objective To investigate the effects of iodine deficiency and hypothyroidism on the expression of cAMP response element binding protein (CREB) in rat cerebellum. Methods Low iodine diet and propylthiouracil (PTU) were used to induce low iodine and hypothyroidism in Wistar rats. The rats were randomly divided into control group, hypothyroidism group (5 mg / LPTU group, 15 mg / LPTU group), and iodine deficiency group, on the 14th day after birth (PN14), PN21, PN28, and PN42, the cerebellum weights of pups were measured and the cerebellar tissues were taken from PN7, PN14, PN21, PN28 and PN42 for immunocytochemistry Avidin-peroxidase (SP) staining was used to analyze the expression of CREB in the cerebellar cortex of rat offspring by image analysis. Results When PN14, PN21, PN28 and PN42, the cerebellar weight of iodine deficiency group was significantly lower than that of control group and hypothyroidism group (P <0.05). PN7, there was no significant difference in the average integral optical density of CREB between the groups, while the average integral optical density values of 5, 15mg / LPTU group and iodine deficiency group at PN14, PN21, PN28 and PN42 were lower than that of the control group P <0.05). Conclusion Iodine deficiency and hypothyroidism can reduce CREB expression in rat cerebellum and affect the development of the nervous system.