一种用于直接测序的ＰＣＲ产物一步纯化法ＰＣＲ产物一般都含有过量的引物和核苷酸，这些物质将直接影响双脱氧ＰＣＲ测序。本方法不同于常规纯化方法，仅仅是在ＰＣＲ产物中加入核酸外切酶Ⅰ（ｅｘｏｎＩ）和来源于虾的碱性磷酸酶，达到纯化的目的。因此，无须多次进行样... A PCR Product for Direct Sequencing One-Step Purification PCR products generally contain excess primers and nucleotides that directly affect dideoxy PCR sequencing. This method is different from the conventional purification method except that exon I and alkaline phosphatase derived from shrimp are added to the PCR product for the purpose of purification. So, do not need to do it multiple times ...