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目的:初步探讨米非司酮抗早孕的作用机制和Notch信号转导通路的关系。方法:采用实时荧光定量逆转录聚合酶链反应技术(TR-qPCR)和免疫组织化学技术检测正常早孕绒毛组织中及不同剂量米非司酮作用后绒毛组织中Notch信号转导通路相关分子Notch-1、Snail、E-钙黏素(E-cadherin)在mRNA水平及蛋白水平的表达情况。结果:米非司酮对早孕绒毛组织的作用以200mg组(D组)最为显著,Notch-1、Snail基因以及E-钙黏素蛋白表达较对照组(A组)、100mg组(B组)、150mg组(C组)作用明显,差异有统计学意义(P<0.05);B组与A组比较差异无统计学意义(P>0.05)。C组Notch-1mRNA、Snail蛋白与A组比较差异有统计学意义(P<0.05),其余各基因及蛋白表达差异均无统计学意义(P>0.05)。结论:米非司酮抗早孕机制可能和Notch信号转导通路有关。
Objective: To investigate the relationship between the mechanism of anti-pregnancy and the mechanism of Notch signal transduction in mifepristone. Methods: The expression of Notch-related signal transduction pathway-related molecules Notch-1 in normal villi tissues and mifepristone were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (TR-qPCR) and immunohistochemistry 1, Snail, E-cadherin mRNA and protein levels in the expression. Results: The effect of mifepristone on the chorionic villi in early pregnancy was the most significant in the 200mg group (group D). The expressions of Notch-1, Snail gene and E-cadherin were higher than those in the control group (group A), 100mg group (group B) , 150mg group (group C), the difference was statistically significant (P <0.05); There was no significant difference between group B and group A (P> 0.05). There was no significant difference in the expression of Notch-1mRNA and Snail between group C and group A (P <0.05). There was no significant difference between other groups (P> 0.05). Conclusion: The anti-pregnancy mechanism of mifepristone may be related to Notch signal transduction pathway.