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本文对人周围血液内的淋巴细胞进行了E-玫瑰花环和EAC玫瑰花环试验及硫代黄素荧光染色。实验证明人周围血液中的大部分淋巴细胞是蓝色核蓝色荧光胞质的小淋巴细胞和蓝色荧光核胞质无荧光而含有蓝白色块状荧光的淋巴细胞。其次是深蓝色核鲜蓝色荧光胞质的淋巴细胞和桔黄色核或桔红色核、黄色荧光胞质的淋巴细胞。此外,有时还可以看到灰蓝色——灰黄色核淡蓝绿色荧光胞质和淡黄色荧光的淋巴细胞。偶而可见桔红色核几乎看不到胞质的淋巴细胞。蓝色较小的细胞核境界不清楚的淋巴细胞和含有蓝白色块状荧光的淋巴细胞及桔黄色核黄色荧光胞质的淋巴细胞与羊红细胞能形成E-玫瑰花环,是T细胞。另一种较小的蓝色荧光的淋巴细胞和深蓝色核、鲜蓝色荧光胞质的淋巴细胞及桔红色核、黄色荧光胞质的淋巴细胞与特异性抗体和补体致敏的羊红细胞能形成EAC玫瑰花环,是B细胞。
In this paper, human peripheral blood lymphocytes were E-rose and EAC rosette test and thioflavin fluorescence staining. Experiments show that most of the human peripheral blood lymphocytes are small nuclear blue blue fluorescent cytoplasm of small lymphocytes and blue fluorescent nuclear cytoplasm without fluorescence and containing blue and white massive fluorescence of lymphocytes. Followed by dark blue nuclear blue fluorescent cytoplasmic lymphocytes and orange or orange nuclear nuclear, yellow fluorescent cytoplasmic lymphocytes. In addition, gray-blueish-yellowish-yellowish-green fluorescent cytoplasm and yellowish-fluorescent lymphocytes are also sometimes seen. Occasionally visible orange nuclei can hardly see the cytoplasm of lymphocytes. Small blue nuclei unclear state of the border cells and contains blue and white massive fluorescent lymphocytes and yellow nuclear yellow fluorescent cytoplasm of lymphocytes and sheep red blood cells can form E-rosette, T cells. Another smaller blue fluorescent lymphocytes and dark blue nucleus, bright blue fluorescent cytoplasmic lymphocytes and orange nuclei, yellow fluorescent cytoplasmic lymphocytes and specific antibodies and complement-sensitized sheep red blood cells can The formation of EAC rose wreath, is a B cell.