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本文报道用不含粘合剂的氧化铝薄层,以二甲苯-丙酮-无水乙醇-二乙胺(50:40:10:0.6)为展开剂分离茄科植物中的莨菪碱、东莨菪碱、山莨菪碱、樟柳碱及红古豆碱等五种莨菪烷生物碱,用改良Dragendorff试剂-Wagner试剂(1:1)显色,定量收集莨菪碱、东莨菪碱及山莨菪碱的斑点,莨菪碱和东莨菪碱用氨甲醇洗脱,蒸干洗脱液后分别用pH 5.6及pH 4.5的溴甲酚绿溶液作比色测定;山莨菪碱用4％醋酐氯仿洗脱,蒸干洗脱液后用pH 5.6的溴甲酚绿溶液作比色测定。本法需要样品量小,提取方法简便,测定方法灵敏稳定、重现性好,适用于多种茄科植物样品及其制剂的分析。
This paper reports the separation of scopolamine, scopolamine from Solanaceae plants using a binder-free alumina thin layer and using xylene-acetone-anhydrous ethanol-diethylamine (50:40:10:0.6) as a developing agent. Five kinds of sterane alkaloids such as anisodamine, anisodine, and red halvedrine were visualized with modified Dragendorff reagent-Wagner reagent (1:1) to collect spots of scopolamine, scopolamine, and anisodamine quantitatively. The scopolamine was eluted with ammonia methanol and evaporated to dryness. The bromocresol green solution at pH 5.6 and pH 4.5 was used for colorimetric determination. Anisodamine was eluted with 4% acetic anhydride and chloroform, and the eluate was evaporated. Colorimetric determinations were made with a bromocresol green solution at pH 5.6. This method requires a small amount of sample, simple extraction method, sensitive and stable determination method, good reproducibility, and is applicable to the analysis of various solanaceous plant samples and their preparations.