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目的探讨直接免疫荧光法快速检测早期甲型流感病毒抗原及其临床应用。方法选择临床上诊断为急性呼吸道感染入院的患者,采集标本200例,将其分为两组:冬春两季流行季节儿童组(100例)和夏秋两季非流感流行期间儿童组(100例),使用美国D3Ultra甲型流感病毒抗原检测试剂,采用荧光标记的单克隆抗体对感冒患者进行甲型流感病毒抗原检测。结果在冬春两季流感流行期间,100例儿童检测有8例阳性,阳性率为8%;在夏秋两季非流感流行期间100例儿童检测有2例阳性,阳性率为2%;两组阳性率比较差异有统计学意义(P<0.05)。结论运用直接免疫荧光法快速检测甲型流感毒感抗原是一种经济、高效、快速、特异的检测方法,能指导临床快速诊断,合理用药。
Objective To investigate the rapid detection of early stage influenza A virus antigen by direct immunofluorescence and its clinical application. Methods A total of 200 patients were randomly divided into two groups: children in winter and spring seasons (100 cases) and children in non-flu epidemic in summer and fall (100 cases ), The use of the United States D3Ultra influenza A antigen detection reagent, the use of fluorescent-labeled monoclonal antibodies to influenza patients influenza A virus antigen detection. Results In the winter and spring epidemics, 100 cases of children were detected positive in 8 cases, the positive rate was 8%; during the non-influenza epidemic in summer and autumn, 100 cases of children were detected in 2 cases, the positive rate was 2%; two groups The positive rate was significantly different (P <0.05). Conclusion The rapid detection of influenza A virus antigen by direct immunofluorescence assay is an economical, efficient, rapid and specific detection method that can guide clinical rapid diagnosis and rational use of drugs.