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采用4h~(51)Cr释放试验及~3H-TdR掺入法,分析γδT细胞识别并杀伤肿瘤细胞的分子机理。主要结果,(1)γδT细胞能够识别多种肿瘤细胞,如XG-7,K562,Daudi,U937,Jurkat等细胞,并可介导高效杀伤作用;(2)γδT细胞亦能对自体及异体新鲜白血病细胞产生高效细胞毒活性;(3)γδT细胞对正常淋巴母细胞无明显杀伤作用;(4)某些细胞因子可协同提高γδT细胞的细胞毒效应,如IL-4,TNF;(5)γδT细胞可高水平分泌IL-2,TNF,γ-IFN等细胞因子;(6)抗热休克蛋白(HSP)70分子单抗可阻断γδT细胞对XG-7细胞的杀伤作用。由此表明,γδT细胞能够特异识别肿瘤抗原,而HSP则参与了肿瘤抗原的递呈及识别过程。
The 4h~(51)Cr release assay and ~3H-TdR incorporation assay were used to analyze the molecular mechanism of γδT cell recognition and tumor cell killing. The main results are as follows: (1) γδ T cells can recognize a variety of tumor cells, such as XG-7, K562, Daudi, U937, Jurkat and other cells, and can mediate their high-level killing effect; (2) γδT cells can also be autologous and allogeneic. Leukemia cells produce highly cytotoxic activity; (3) γδT cells have no apparent killing effect on normal lymphoblasts; (4) Some cytokines can synergistically increase the cytotoxic effects of γδT cells, such as IL-4 and TNF; (5) γδT cells can secrete IL-2, TNF, γ-IFN and other cytokines at high levels. (6) The heat shock protein 70 (HSP) 70 monoclonal antibody can block the killing effect of γδT cells on XG-7 cells. This shows that γδT cells can specifically recognize tumor antigens, while HSP is involved in the process of tumor antigen presentation and recognition.