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给RF/J品系小鼠注射甲基亚硝基尿(MNU,N-Nitroso-N-Methylutea)每周30mg/kg,共5周。全部小鼠都发生T细胞淋巴瘤。为了搞清楚我们在早期肿瘤发生与发展的研究中所观察到的染色体非随机变化的意义,我们仔细观察了其原发性淋巴瘤及其在裸鼠和同基因小鼠中连续传代培养细胞的染色体。结果表明,早期的绝大多数变异是染色体数目差异,仅少数为结构变化。虽然在全部小鼠中受累的染色体不完全相同,但是常常局限在某些染色体上,主要是X, l, 3, 4, 6, 10, 12和15染色体,其出现受累的频率在受检细胞中达5—47％。绝大多数发生变化的染色体在后来的传代培养中持续出现,但有少数丢失,亦有频率增加和出现新的变化等现象。这与其他鼠类白血病和淋巴细胞增生性疾病所观察到的结果是一致的。MNU有效地产生这些变化的能力而使其出现高度的组织特异性和有效的致癌作用,可能是其对T细胞染色体转化的优先选择作用所致。
The mice of RF/J strain were injected with 30 mg/kg of MNU, N-Nitroso-N-Methylutea weekly for 5 weeks. All mice developed T-cell lymphoma. In order to understand the significance of the non-random changes in chromosomes that we observed in the study of early tumorigenesis and development, we carefully observed its primary lymphomas and their continuous subculture of cells in nude mice and syngeneic mice. chromosome. The results showed that the vast majority of the early variants were differences in chromosome numbers, and only a few were structural changes. Although the chromosomes involved in all mice are not identical, they are often confined to certain chromosomes, mainly X, l, 3, 4, 6, 10, 12 and 15 chromosomes, and the frequencies of their appearance are affected by the examined cells. Up to 5 to 47%. The vast majority of chromosomes that have changed have continued to appear in subsequent subcultures, but a few have been lost, and there have been frequency increases and new changes. This is consistent with the observed results from other murine leukemia and lymphoproliferative diseases. The ability of MNU to effectively produce these changes makes it highly tissue-specific and potent oncogenic, possibly due to its preferential effect on chromosomal transformation of T cells.