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用不同浓度2-氨基磷酸茚(AIP),并结合茉莉酸甲酯(Me JA)处理新疆紫草悬浮细胞,测定不同处理组、不同处理时间细胞中迷迭香酸及乙酰紫草素、脱氧紫草素、β,β’-二甲基丙烯酰紫草素、异戊酰紫草素等次生代谢物的含量,考察AIP抑制苯丙氨酸通路后对紫草悬浮细胞中次生代谢物合成、积累的影响。结果显示Me JA处理能够明显促进新疆紫草细胞中次生代谢物的合成积累;AIP处理能够抑制新疆紫草细胞中上述次生代谢物的积累,并在二者联合处理时能一定程度上抵消Me JA的促进作用,且抑制作用的强弱与处理剂量、处理时间存在正相关性。表明苯丙氨酸途径在紫草素类化合物生物合成中具有重要作用,可为通过细胞培养方式生产紫草素类化合物的代谢调控及紫草素类化合物生物合成等进一步研究提供参考。
Different concentrations of 2-aminoindrine (AIP) and methyl jasmonate (Me JA) were used to treat the suspension cells of Arnebia euchroma (Royle) Johnston and the determination of rosmarinic acid and acetylshikonin in different treatment groups, Shikonin, β, β’-dimethacrylshikonin, isovaleryl shikimicin and other secondary metabolites content of AIP inhibition phenylalanine pathway on the secondary metabolites of Lithospermum cells Material synthesis, the impact of accumulation. The results showed that Me JA treatment could significantly promote the accumulation of secondary metabolites in Lithospermum cells in Xinjiang. AIP treatment could inhibit the accumulation of the above secondary metabolites in Lithospermum cells in Xinjiang and could counteract to some extent when combined treatment Me JA promote the role, and the inhibitory effect of the strength and dose, treatment time there is a positive correlation. It is indicated that the phenylalanine pathway plays an important role in the biosynthesis of shikonin and may provide references for the further research on the regulation of shikonin production by cell culture and the biosynthesis of shikonin.