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目的探讨大鼠实验性牙齿移动中不同正畸力对牙槽骨核因子κB受体活化因子配基(RANKL)表达的影响。方法本研究于2010年9—12月在山西医科大学寄生虫实验室完成。选用健康雄性Wistar大鼠36只,随机分为对照组、轻力组、中力组、大力组(各9只),分别施加0、0.29、0.49、0.98N正畸力,建立大鼠牙齿移动模型。免疫组化法检测大鼠牙周组织压力侧RANKL的表达。结果对照组RANKL有少量表达,主要分布于破骨细胞的胞核,正畸加力各组RANKL表达主要分布于压力侧牙周膜及骨改建区的破骨细胞,在基质细胞和牙周膜成纤维细胞也有表达,且其黄染程度强于对照组。各实验组牙周组织压力侧不同程度地出现血管被挤压、玻璃样变区和骨吸收陷窝。正畸力与RANKL的表达呈正相关(r=0.834,P<0.01)。结论正畸力与大鼠牙槽骨压力侧RANKL的表达呈正相关,且0.49N为促进RANKL表达的最佳力值。
Objective To investigate the effects of different orthodontic forces on the expression of nuclear factor κB receptor activator of transcription factor RANKL in experimental tooth movement in rats. Methods The study was performed in the Parasite Laboratory of Shanxi Medical University from September to December in 2010. Thirty-six healthy male Wistar rats were randomly divided into control group, mild stress group, moderate stress group, and Dali treatment group (n = 9 each). Orthodontic force of 0, 0.29, 0.49 and 0.98 N were applied to establish rat tooth movement model. Immunohistochemistry was used to detect the expression of RANKL in the periodontal tissues of rats. Results The expression of RANKL in the control group was small, mainly distributed in the nucleus of osteoclasts. The expression of RANKL in orthodontic force groups was mainly distributed in the periodontal ligament of the pressure side and the osteoclasts in the bone remodeling area. In the stromal cells and periodontal ligament Fibroblasts were also expressed, and the degree of yellow dye than the control group. The experimental group of periodontal tissue pressure side of the vessel appears to varying degrees, being squeezed, glassy area and bone resorption lacuna. Orthodontic force was positively correlated with RANKL expression (r = 0.834, P <0.01). Conclusion Orthodontic force is positively correlated with the expression of RANKL on the pressure side of alveolar bone in rats, and 0.49N is the best value for promoting RANKL expression.