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利用多基因型集成品种群体(由多个类似表型的单基因型品种或品系组成)和单基因型品种群体,分析两种群体中的稻瘟菌群体遗传结构及发病情况。将这两种群体植株上所采集标样的菌株进行分离纯化,应用细菌基因组重复序列PCR技术(rep-PCR)获得各菌株指纹图谱,并将其划分为若干单元型进行多样性分析。结果显示,稻瘟菌多样性指数范围在集成品种群体内为0.521～0.603,在单一品种群体内为0.319～0.705,说明两种群体内的病菌多样性没有差异,稻瘟菌多样性在抗性弱的品种内低,抗性强的品种内高。病害调查结果表明,集成品种的实际病穗率低于预期发病率,差异极显著。由于集成品种群体中含有抗性强材料阻止了致病菌的扩散,从而控制了病害。进一步证实了种植集成品种是一条控制稻瘟病的有效途径。
The genetic structure and pathogenesis of two populations of Magnaporthe grisea were analyzed using the integrated multi-genotype breed (consisting of more than one single-genotype or lineage of similar phenotype) and single-genotype breeds. The strains collected from these two populations were isolated and purified. The fingerprints of each strain were obtained by rep-PCR and divided into haplotypes for diversity analysis. The results showed that the diversity index of Magnaporthe grisea ranged from 0.521 to 0.603 in the integrated population and from 0.319 to 0.705 in the single population, indicating that there is no difference in the diversity of pathogenic bacteria in the two populations. The diversity of Magnaporthe grisea is weak in resistance Within the varieties of low, high resistance varieties. The results of disease survey showed that the actual disease panicle rate of integrated varieties was lower than the expected incidence, the difference was extremely significant. The disease is controlled by the presence of resistant material in the integrated breed population that prevents the spread of pathogenic bacteria. It is further confirmed that planting integrated varieties is an effective way to control rice blast.