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目的:探讨异种异体半月板移植对延缓关节软骨退变的作用。方法:对15只成年新西兰兔的后肢左、右膝均进行内侧半月板全切。在15只兔的左膝进行猪半月板组织成型后所得的异种异体内侧半月板移植,以其已切除内侧半月板的右膝作为对照。分别于术后6周、12周、24周择期处死动物,观察各组兔膝关节软骨的大体病理表现、组织病理Mankin评分改变,并用Ⅰ、Ⅱ、Ⅲ、Ⅹ型胶原单克隆抗体免疫组织化学染色法观察关节软骨中Ⅰ、Ⅱ、Ⅲ、Ⅹ型胶原的表达情况。结果:兔内侧半月板全切组膝内侧胫骨平台和内侧股骨髁软骨破坏严重,有大量骨赘形成,呈骨性关节炎表现。异种异体半月板移植在短期内对关节软骨具有明显保护作用,但随着术后24周移植半月板被破坏,关节软骨出现退变,但与同期的半月板切除膝相比,关节软骨退变较轻。结论:异种异体半月板移植短期内对关节软骨有保护作用;异种异体半月板移植可延缓关节软骨退行性变。
Objective: To investigate the effect of allogeneic meniscus transplantation on delaying the degeneration of articular cartilage. Methods: The left and right knees of 15 adult New Zealand rabbits were all medial meniscectomy. Allogeneic medial meniscus transplantation was performed on the left knee of 15 rabbits after the meniscal tissue was formed. The right knee of the medial meniscus was resected as the control. The animals were sacrificed at 6, 12 and 24 weeks postoperatively, respectively. The gross pathological changes of knee cartilage were observed and the Mankin score of histopathological changes were observed. Immunohistochemistry with type I, II, III and X collagen monoclonal antibodies The expression of collagen type Ⅰ, Ⅱ, Ⅲ and Ⅹ in articular cartilage was observed by staining method. Results: The medial tibial plateau and the medial femoral condyle cartilage were severely destroyed in the medial meniscectomy group. There was a large amount of osteophyte formation, showing osteoarthritis. Allogeneic meniscus transplantation in the short term has significant protective effect on articular cartilage, but with the destruction of the transplanted meniscus after 24 weeks, articular cartilage degeneration, but compared with the same period of meniscus resection knee, articular cartilage degeneration Lighter. CONCLUSION: Allogeneic meniscus transplantation has a protective effect on articular cartilage in a short term. Allogeneic meniscus transplantation can delay the degeneration of articular cartilage.