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目的:建立固相萃取-高效液相色谱法,同时测定伤疡愈软膏中紫草的主要成分β,β’-二甲基丙烯酰阿卡宁和丹参中丹酚酸B的含量。方法:采用固相萃取法和高效液相色谱法联用,选用C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-水-甲醇-甲酸(700∶300∶30∶15)为流动相;流速:1.0 ml·min~-1;β,β’-二甲基丙烯酰阿卡宁检测波长:275 nm,丹酚酸B检测波长为286 nm的相同系统下同时测定伤疡愈软膏中紫草的主要成分β,β’-二甲基丙烯酰阿卡宁和丹参中丹酚酸B的含量。结果:β,β’-二甲基丙烯酰阿卡宁浓度在23.780~118.900μg·ml-1范围内线性关系良好(r=0.999 5),回收率为99.5%,RSD=1.07%;丹酚酸B在19.840~99.200μg·ml-1范围内线性关系良好r=0.999 3,丹酚酸B的平均回收率为98.2%,RSD=2.1%(n=9)。结论:该方法操作简单,快速,分离度好,建立的定量方法可用于伤疡愈软膏的质量控制。
OBJECTIVE: To establish a solid-phase extraction-high performance liquid chromatography (HPLC) method for the simultaneous determination of salvianolic acid B and salidroside B β in Rhus chinensis. Methods: Solid phase extraction and high performance liquid chromatography (HPLC) were used with C18 (250 mm × 4.6 mm, 5 μm) column. The mobile phase was acetonitrile - water - methanol - ; Flow rate: 1.0 ml · min ~ -1; β, β’-dimethylacryloxanamycin detection wavelength: 275 nm, salvianolic acid B detection wavelength of 286 nm in the same system Simultaneous determination of ulcer healing ointment The main components of Lithospermum β, β’-dimethylacryloracan and salvia in Salvianolic acid B content. Results: The linear range was 23.780 ~ 118.900μg · ml-1, the recovery was 99.5% and the RSD was 1.07% The linear relationship of acid B in the range of 19.840 ~ 99.200μg · ml-1 was good, r = 0.999 3. The average recovery of salvianolic acid B was 98.2% with RSD = 2.1% (n = 9). Conclusion: The method is simple, rapid, and good in separation. The established quantitative method can be used for the quality control of Yuyao Yuzhi.