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应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠用绵羊红细胞（ＳＲＢＣ）免疫后２～７天期间，脾脏和胸腺中去甲肾上腺素（ＮＡ）、５－羟色胺（５－ＨＴ）和５－羟吲哚乙酸（５－ＨＩＡＡ）含量的变化。结果表明，脾脏和胸腺中ＮＡ含量在免疫后第４和第５天均显著减少；脾脏中５－ＨＴ水平在第３和第４天明显升高，５－ＨＩＡＡ含量在第５和第６天明显增多；胸腺内５－ＨＴ含量仅在免疫后第６天显著增加，５－ＨＩＡＡ水平没有明显变化。以上结果提示，体液免疫应答可影响淋巴器官内单胺类递质的代谢。结合我们以前的研究结果：ＮＡ可影响脾脏Ｔ细胞的增殖，以及外周５－ＨＴ可抑制体液免疫应答，说明单胺类递质与免疫系统之间存在着相互作用。
The levels of norepinephrine (NA) and serotonin (5-HT) in the spleen and thymus were determined by HPLC-ECD after 2 to 7 days of immunization with sheep red blood cells (SRBC) HT) and 5-hydroxyindoleacetic acid (5-HIAA). The results showed that the content of NA in spleen and thymus decreased significantly on the 4th and 5th day after immunization. The 5-HT level in the spleen increased obviously on the 3rd and 4th day, and the content of 5-HIAA on the 5th and 6th day The content of 5-HT in the thymus increased significantly only on the 6th day after immunization, but the level of 5-HIAA did not change significantly. The above results suggest that the humoral immune response may affect the metabolism of monoamine neurotransmitters in lymphoid organs. Combined with our previous findings: NA can affect spleen T cell proliferation, and peripheral 5-HT can inhibit the humoral immune response, indicating that there is an interaction between monoamine neurotransmitters and the immune system.