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目的建立基于荧光探针的汞离子快速检测方法。方法基于汞离子促进硫羰基脱硫的不可逆反应,设计合成了含有双硫羰基的荧光探针HgP1。通过紫外-可见光谱法和荧光光谱法研究了探针HgP1在甲醇水溶液中对金属离子的识别特性,并探讨了其识别机制。结果探针HgP1对Hg~(2+)具有高效专一的选择性,具有较强的抗金属阳离子和阴离子干扰能力,并能通过溶液颜色变化实现对汞离子的裸眼识别。Hg~(2+)浓度在0.01~0.1μmol/L范围内与该探针溶液荧光发射强度值间呈良好的线性关系(R~2=0.988),该方法对汞离子的最低检测限为0.256μg/kg。结论 HgP1探针对汞离子具有高效专一的选择性,其最低检测限满足国家标准对食品中汞离子的限量要求,具备较强的实际应用价值。
Objective To establish a rapid mercury ion detection method based on fluorescent probe. Methods Based on the irreversible reaction of mercuric ion to promote thiourea desulfurization, a fluorescent probe HgP1 containing dithiocarbonyl was designed and synthesized. The recognition properties of probe HgP1 in metal aqueous solution were studied by UV-Vis spectroscopy and fluorescence spectroscopy, and its recognition mechanism was discussed. Results The probe HgP1 was highly selective to Hg 2+ and had strong anti-metal cation and anion interference ability. The HgP1 probe could recognize the naked eye of mercury ion through the color change of solution. The linear relationship between the concentration of Hg 2+ in the range of 0.01 ~ 0.1 μmol / L and the fluorescence intensity of the probe solution (R ~ 2 = 0.988) was obtained. The detection limit of mercury ion was 0.256 μg / kg. Conclusion The HgP1 probe has high efficiency and specificity for mercury ion selectivity. The minimum detection limit of HgP1 probe meets the national standard limit of mercury ion in food, which has strong practical value.