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目的：探讨ＩＬ７对纯化瘤性Ｔ细胞群生长信号的刺激作用。白细胞介素（ＩＬ）７能刺激一些良性和恶性淋巴细胞群，包括来自皮肤Ｔ细胞淋巴瘤（ＣＴＣＬ）病人的肿瘤细胞。由于角化细胞产生ＩＬ７，因而ＩＬ７可能在ＣＴＣＬ和其他炎性皮肤病的生物学上起作用。方法：细胞从ＣＴＣＬ患者外周血中分离，体外ＩＬ７处理１～１０ｍｉｎ，ＤＮＡ合成法检测细胞的增殖。应用免疫沉淀和Ｗｅｓｔｅｒｎ印迹技术，研究酪氨酸激酶类对ＩＬ７的信号应答。结果：与正常Ｔ细胞不同的是，在无丝裂原下用ＩＬ７体外培养的ＣＴＣＬ细胞增殖。ＩＬ７孵育之后细胞内不同底物快速发生酪氨酸磷酸化。ｇｅｎｉｓｔｅｉｎ能抑制其细胞增殖和酪氨酸磷酸化。Ｓｒｃ家族蛋白之一酪氨酸激酶ｐ５９ｆｙｎ，在ＩＬ７处理１ｍｉｎ内发生酪氨酸磷酸化，而作为酪氨酸磷酸化可能候选的ＰＩ３激酶的ｐ８５亚基并未发生。结论：提示ＩＬ７诱导酪氨酸蛋白激酶ｐ５９ｆｙｎ是其介导信号传导途径的早期事件，且在ＣＴＣＬ的病理生理学中起重要作用。
Objective: To investigate the IL 7 on the growth of purified tumor T cell population stimulation signal. Interleukin (IL) 7 can stimulate a number of benign and malignant lymphocyte populations, including tumor cells from cutaneous T-cell lymphoma (CTCL) patients. Since keratinocytes produce IL-7, IL-7 may play a role in the biology of CTCL and other inflammatory skin diseases. Methods: The cells were isolated from the peripheral blood of patients with CTCL, in vitro IL 7 treatment 1 ~ 10min, DNA synthesis assay of cell proliferation. Immunoprecipitation and Western blotting were used to study the signal response of tyrosine kinases to IL-7. Results: Unlike normal T cells, CML cells cultured in vitro without mitogens proliferated. Tyrosine phosphorylation occurs rapidly on different substrates in IL-7 after incubation. Genistein can inhibit its cell proliferation and tyrosine phosphorylation. Tyrosine kinase p59fyn, one of the Src family proteins, undergoes tyrosine phosphorylation within 1 min of IL-7 treatment, whereas the p85 subunit of PI-3 kinase, which may be a candidate for tyrosine phosphorylation, does not occur. Conclusion: It is suggested that IL7-induced tyrosine protein kinase p59fyn is an early event that mediates signal transduction pathway and plays an important role in the pathophysiology of CTCL.