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以糖尿病小鼠代谢指标和肌肉Glut4、PI3K、Akt1和Akt2基因表达为观测点,探讨巴氏蘑菇多糖(Agaricus blazei Murill polysaccharides,ABMP)的降血糖作用。体重(30±2)g的健康雄性小鼠随机分为:正常对照组(NC组)、高血糖模型组(HM组)、低剂量组(LD组)、中剂量组(MD组)、高剂量组(HD组)、药物治疗组(DT组),每组8只。HM组、LD组、MD组、HD组和DT组腹腔注射2%四氧嘧啶3 d(200 mg·kg~(-1)体重),禁食12 h后,血糖值高于11 mmol·L~(-1)说明建模成功。NC组和HM组每天给予0.9%生理盐水,LD组、MD组、HD组每天分别给予50 mg/kg·体重、100 mg/kg·体重、200 mg/kg·体重的ABMP,DT组每天给予200 mg/kg·体重的阿卡波糖。每周测1次体重,7周后,检测血糖、胰岛素、肝糖原含量和肌肉组织中Glut4、PI3K、Akt1和Akt2基因的表达量。结果表明,与NC组相比,HM组空腹血糖升高,差异显著(P<0.01),血清胰岛素和肝糖原水平明显降低(P<0.01),肌肉中Glut4、PI3K、Akt1和Akt2基因的表达量下降,差异具有统计学意义(P<0.05)。与HM组相比,LD组、MD组、HD组和DT组空腹血糖值均下降,差异显著(P<0.05),血清胰岛素和肝糖原含量均上升,差异达显著水平(P<0.05);肌肉中Glut4、PI3K、Akt1和Akt2基因的表达量升高。ABMP能够调节糖尿病小鼠体内糖代谢,降低血糖。
The hypoglycemic effect of Agaricus blazei murill polysaccharides (ABMP) was investigated using the metabolic indices of diabetic mice and the expressions of muscle Glut4, PI3K, Akt1 and Akt2 genes. Healthy male mice weighing 30 ± 2 g were randomly divided into normal control group (NC group), hyperglycemia model group (HM group), low dose group (LD group), middle dose group (MD group), high Dose group (HD group) and drug treatment group (DT group), 8 in each group. The rats in HM group, LD group, MD group, HD group and DT group were intraperitoneally injected with 2% alloxan for 3 days (200 mg · kg -1 body weight). After fasting for 12 hours, the blood glucose level was higher than 11 mmol·L ~ (-1) Description Modeling success. The rats in NC group and HM group were given 0.9% saline every day, LD group, MD group and HD group were administrated with ABMP of 50 mg / kg · body weight, 100 mg / kg · body weight and 200 mg / kg · body weight every day, 200 mg / kg body weight of acarbose. The body weight was measured weekly, and the levels of blood glucose, insulin, hepatic glycogen and the expression of Glut4, PI3K, Akt1 and Akt2 genes in muscle tissue were measured 7 weeks later. The results showed that compared with NC group, fasting blood glucose in HM group increased significantly (P <0.01), serum insulin and hepatic glycogen level decreased significantly (P <0.01), while Glut4, PI3K, Akt1 and Akt2 The expression decreased, the difference was statistically significant (P <0.05). Compared with HM group, the fasting blood glucose levels of LD group, MD group, HD group and DT group decreased significantly (P <0.05), and the levels of serum insulin and hepatic glycogen increased significantly (P <0.05) ; Muscle Glut4, PI3K, Akt1 and Akt2 gene expression increased. ABMP can regulate glucose metabolism in diabetic mice and lower blood sugar.