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目的观察不同输液量救治失血性休克的疗效及其脑组织AQP4的表达。方法家兔28只,左颈总动脉放血建立失血性休克模型,随机分成单纯休克组(A)、常规输液组(B)、大量输液组(C)、正常对照组(D),动态监测血压、心电图和呼吸,HE和AQP4免疫组化(IHC)染色,图像分析技术定量检测各组脑组织间隙相对面积和AQP4表达分布情况。结果 A组死亡率(57.1%)低于C组(85.7%),但两者差异无统计学意义(P>0.05);而B组无死亡。C组脑组织苍白肿胀,HE染色神经元及胶质细胞高度水变性,间质明显疏松,血液稀释、血浆色淡,组织间隙相对面积最大14.83%±8.01%。IHC染色,与B、D组比较,A、C组脑组织AQP4表达明显增多,阳性着色深。定量检测和方差分析各组累积光密度(IOD)差异有统计学意义,P<0.05。结论正常输液量救治休克疗效好,而过量输液死亡率高与脑组织水分过多清除障碍,引起的生命中枢功能障碍有关。
Objective To observe the curative effect of hemorrhagic shock with different infusion volume and the expression of AQP4 in brain tissue. Methods 28 rabbits were sacrificed and the left common carotid artery was sacrificed to establish a hemorrhagic shock model. The rats were randomly divided into shock group (A), conventional infusion group (B), transfusion group (C), normal control group (D) , Electrocardiogram (ECG) and respiration, HE and AQP4 immunohistochemistry (IHC) staining and image analysis technique were used to quantitatively detect the relative area of brain interstitial space and the distribution of AQP4 in each group. Results The mortality in group A (57.1%) was lower than that in group C (85.7%), but there was no significant difference between the two groups (P> 0.05). The brain tissue of group C had pale swelling, the HE-stained neurons and glial cells were highly degenerated. The interstitial space was obviously loosened. The relative area of hemodilution, pale color and interstitial space was 14.83% ± 8.01%. IHC staining, compared with B, D group, A, C group AQP4 brain tissue expression increased significantly positive staining depth. Quantitative detection and analysis of variance in each group accumulated optical density (IOD) difference was statistically significant, P <0.05. Conclusion The normal infusion rate of shock treatment is good, and excessive infusion of high mortality and brain tissue excessive water removal disorders, caused by the central dysfunction of life.