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目的探讨姜黄素对乳腺癌细胞增殖、迁移及侵袭能力的影响及其作用机制。方法体外培养乳腺癌细胞MDA-MB-231,对照组分别给予二甲基亚砜(DMSO)10μL,处理48 h,低、中、高3个剂量实验组分别给予5,10,20μg·m L~(-1)姜黄素,处理48 h。用四甲基偶氮唑盐(MTT)法检测乳腺癌细胞增殖能力的变化,用TUNEL法检测乳腺癌细胞凋亡的程度;用划痕实验及Transwell侵袭实验分别检测乳腺癌细胞的迁移及侵袭能力,用Western Blot法检测相关蛋白表达水平。结果处理48 h后,对照组和中、高2个剂量实验组的细胞增殖能力分别为100.00%,67.63%和49.17%,细胞凋亡率分别为0.38%,6.44%和24.22%,细胞迁移能力分别为100.00%,55.33%和40.67%,侵袭细胞数为(104±8),(37±7),(25±9)个,磷酸化P38蛋白表达量分别为100.00%,57.63%和42.51%,对照组和中、高2个剂量实验组比较差异均有统计学意义(均P<0.05)。结论姜黄素能够降低P38磷酸化水平,从而抑制乳腺癌细胞的增殖、迁移及侵袭能力,抑制其凋亡,其机制有待进一步研究。
Objective To investigate the effect of curcumin on the proliferation, migration and invasion of breast cancer cells and its mechanism. Methods The breast cancer cells MDA-MB-231 were cultured in vitro. The control group was given DMSO (DMSO) 10μL for 48 hours. The low, middle and high dose groups were given 5,10,20μg · m L ~ (-1) curcumin for 48 h. The proliferation of breast cancer cells was detected by MTT assay. The extent of apoptosis of breast cancer cells was detected by TUNEL assay. Scratch and Transwell invasion assays were used to detect the migration and invasion of breast cancer cells Ability, using Western Blot test related protein expression level. Results After 48 h of treatment, the cell proliferation ability of the control group and middle and high dosage groups were 100.00%, 67.63% and 49.17% respectively, and the apoptosis rates were 0.38%, 6.44% and 24.22% respectively. The cell migration ability (104 ± 8), (37 ± 7) and (25 ± 9), respectively. The phosphorylated P38 protein expression levels were 100.00%, 57.63% and 42.51% respectively, which were 100.00%, 55.33% and 40.67% , There was significant difference between the control group and middle and high dose two experimental groups (all P <0.05). Conclusion Curcumin can reduce the level of P38 phosphorylation, thereby inhibiting the proliferation, migration and invasion ability of breast cancer cells and inhibiting its apoptosis. The mechanism remains to be further studied.