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目的：克隆人血小板生成素基因，方法：采用ＲＴ一ＰＣＲ的方法。结果：从人胎肝中克隆出全长的血小板生成素（ＴＰＯ）基因，并亚克隆至ＰＵＣｌｌ８载体中。该ｃＤＮＡ全长１０６２ＢＰ，编码３５３个氨基酸，经序列测定与Ｂａｒｔｌｅｙ等人报道的完全一致［１］。结论：已获得人血小板生成素基因，此项工作为基因工程生产ＴＰＯ奠定了良好基础。
Objective: To clone human thrombopoietin gene, Methods: RT-PCR method. Results: Full-length thrombopoietin (TPO) gene was cloned from human fetal liver and subcloned into PUC118 vector. The full-length cDNA of 1062BP, encoding 353 amino acids, was sequenced in accordance with Bartley et al. [1]. Conclusion: The human thrombopoietin gene has been obtained. This work laid a good foundation for genetically engineered TPO production.