为了研究镍的生殖毒性 ,采用卵母细胞体外培养、体外受精的方法研究镍对小鼠卵母细胞的成熟和体外受精率的影响。结果 :氯化镍对卵母细胞生发泡破裂没有影响 ,但抑制卵母细胞第一极体的排放 ,降低体外受精率 ,并降低卵母细胞的存活率。实验结果提示 ,氯化镍破坏小鼠卵母细胞的减数分裂 ,影响小鼠的生殖功能。 In order to study the reproductive toxicity of nickel, the effects of nickel on mouse oocyte maturation and in vitro fertilization rate were studied by oocyte in vitro culture and in vitro fertilization. RESULTS: Nickel chloride had no effect on oogenesis rupture of oocytes, but inhibited the discharge of the first polar body of oocytes, decreased in vitro fertilization rate and decreased the oocyte survival rate. The experimental results suggest that nickel chloride destroys the meiosis of mouse oocytes and affects the reproductive function of mice.