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目的:研究赤雹根总皂苷(STDR)对类风湿性关节炎(RA)大鼠足跖组织中核转录因子(NF-κB)p65 mRNA、白介素6(IL-6)mRNA、白介素10(IL-10)mRNA相对表达水平的影响。方法:雄性SD大鼠80只,随机取10只大鼠作为正常对照,其余大鼠采用弗氏完全佐剂诱导佐剂性关节炎,将造摸成功的50只大鼠随机分为模型组,赤雹根总皂苷160,80,40 mg·kg-1·d-1组和雷公藤多苷12 mg·kg-1·d-1组,各组分别给予相应药物,模型组ig蒸馏水,连续21 d。RT-PCR法检测足跖组织中NF-κB p65 mRNA,IL-6 mRNA,IL-10 mRNA表达水平。结果:与对照组比较,模型组NF-κB p65和IL-6的表达显著增高(P<0.01),IL-10的表达明显降低(P<0.01);STDR 40,80,160 mg·kg-1剂量组大鼠足跖组织中NF-κB p65 mRNA,IL-6 mRNA相对表达水平均显著低于模型对照组(P<0.01);STDR各剂量组大鼠足跖组织中IL-10 mRNA相对表达水平均显著高于模型对照组(P<0.01)。结论:调节NF-κB p65 mRNA,IL-6 mRNA和IL-10 mRNA表达水平是STDR治疗RA的作用机制之一。
Objective: To investigate the effects of total glucocorticoid (STDR) on nuclear factor-kappa B (NF-κB) p65 mRNA, interleukin 6 (IL-6) 10) mRNA relative expression levels. Methods: Eighty male Sprague-Dawley rats were randomly selected as the normal control. The other rats were given complete Freund’s adjuvant to induce adjuvant arthritis. Fifty successful rats were randomly divided into model group, In the groups of 160, 80, 40 mg · kg-1 · d-1 and 12 mg · kg-1 · d-1 tripterygium glycosides, the rats in each group were treated with distilled water, 21 d. The expression of NF-κB p65 mRNA, IL-6 mRNA and IL-10 mRNA in plantar tissues were detected by RT-PCR. Results: Compared with the control group, the expression of NF-κB p65 and IL-6 in the model group was significantly increased (P <0.01) and the expression of IL-10 was significantly decreased (P <0.01) The relative expression levels of NF-κB p65 mRNA and IL-6 mRNA in the plantar tissues of rats in the three groups were significantly lower than those in the model control group (P <0.01). The relative expression levels of IL-10 mRNA in the plantar tissues Were significantly higher than the model control group (P <0.01). Conclusion: Regulation of NF-κB p65 mRNA, IL-6 mRNA and IL-10 mRNA expression is one of the mechanisms of STDR in the treatment of RA.