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目的:研究哺乳动物雷帕霉素靶蛋白(mTOR)抑制剂CCI-779对激素非依赖型前列腺癌PC-3细胞株化疗敏感性的影响。方法:前列腺癌PC-3细胞培养至对数生长期,CCI-779、紫杉醇单独及联合给药,对照组中加入等体积的培养液;MTT法检测3组药物对PC-3的生长抑制作用,流式细胞术(FCM)检测细胞周期的改变和凋亡率。结果:MTT法显示,与对照组相比,各组药物均可显著抑制PC-3细胞增殖,联合用药能显著增强这种抑制效果;FCM检测发现CCI-779和紫杉醇使PC-3细胞主要阻滞在G1-S期,而联合用药使PC-3细胞主要阻滞在G0/G1期;与对照组相比,药物组PC-3细胞凋亡率均显著增加(P<0.01)。结论:mTOR抑制剂CCI-779能抑制PC-3细胞增殖,增加对前列腺癌的化疗敏感性。
Objective: To investigate the effect of mammalian rapamycin target protein (mTOR) inhibitor CCI-779 on the chemosensitivity of hormone independent prostate cancer cell line PC-3. Methods: Prostate cancer PC-3 cells were cultured in the logarithmic growth phase, CCI-779 and paclitaxel alone and in combination, and the control group was added with equal volume of culture medium. The growth inhibition of PC-3 was assayed by MTT assay , Flow cytometry (FCM) to detect cell cycle changes and apoptosis rate. Results: MTT assay showed that compared with the control group, the drugs of all groups could significantly inhibit the proliferation of PC-3 cells and the combination therapy could significantly enhance this inhibitory effect. FCM showed that CCI-779 and paclitaxel caused the main resistance of PC-3 cells However, the combination of PC-3 cells and G0 / G1 phase delayed the G1-S phase arrest. Compared with the control group, the apoptosis rate of PC-3 cells was significantly increased (P <0.01). Conclusion: The mTOR inhibitor CCI-779 can inhibit the proliferation of PC-3 cells and increase the chemosensitivity to prostate cancer.