The oculomotor nerves of beagle dogs received electrical stimulation at 0.3-2.0 V. After recording compound muscle action potentials of the inferior oblique muscle, the oculomotor nerve was quickly cut off and a direct end-to-end anastomosis was then performed. As a result, the stimulating elec-trode was smoothly inserted and placed, and ideal bioelectrical signals of the interior oblique muscle were acquired. After oculomotor nerve injury, compound muscle action potentials of the inferior oblique muscle were significantly decreased in beagle dogs. These findings suggest that an animal model of oculomotor nerve injury was successfully established for electrophysiological studies. The oculomotor nerves of beagle dogs received electrical stimulation at 0.3-2.0 V. After recording compound muscle action potentials of the inferior oblique muscle, the oculomotor nerve was quickly cut off and a direct end-to-end anastomosis was then performed. , the stimulating elec-trode was bent inserted and placed, and ideal bioelectrical signals of the interior oblique muscle were acquired. After oculomotor nerve injury, compound muscle action potentials of the inferior oblique muscle were significantly less in beagle dogs. These findings suggest an an animal model of oculomotor nerve injury was successfully established for electrophysiological studies.